Anti-Caspase 1 (Interleukin-1Beta Converting Enzyme, ICE)

Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20kD (p20) and a 10kD (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 5ug/ml. , Immunocytochemistry (paraffin): 1:1000 , Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb, I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb, I1904-40D: IgG, H&L (FITC) Pab Gt x Rb, I1904-46N: IgG, H&L (FITC) Pab Gt x Rb, I1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb, Positive Control: THP cell lysate , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.