Anti-BAFF (BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4) (APC)

BAFF (also known as TALL1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 amino acid (aa) protein consisting of a 248aa extracellular domain, a 21aa transmembrane region and a 45 aacytoplasmic tail. BAFF has the typical structural characteristics of TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain. BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS, and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells using mouse splenocytes, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.