Anti-ASAHL (N-Acylsphingosine Amidohydrolase-like)

ASAHL (N-acylsphingosine-amidohydrolase-like, N-acylethanolamine-hydrolyzine acid amidase, also acid ceramidase-like protein) is a 30kD member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30-31kD, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324-359, while another has a two aa substitution for aa 198-359. The human proform (aa 29-359) is 78% aa identical to mouse ASAHL proform. Applications: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhASAHL is ~ 1ng/well., Western Blot: 0.1-0.2ug/ml detection limit for rhASAHL is ~ 10ng/lane under non-reducing and reducing conditions. , Immunoprecipitation: , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.