Anti-ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Adipocyte

Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18kD cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml, Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.