Anti-15-Lipoxygenase-2

Two types of 15-LO have been discovered and characterized, both of which metabolize arachidonic acid (AA) to produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). 15-LO-1 oxygenates AA at both C15 and C12, whereas 15-LO-2 exclusively oxygenates C15 of AA. Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1. Expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues. The protein levels and enzymatic activity of 15-LO-2 are both down-regulated in prostate cancer compared with normal and benign prostate tissues, implicating a possible protective role for 15-LO-2 against tumor formation. Cayman Chemical's 15-LO-2 polyclonal antibody can be used for western blot analysis for 15-LO-2 on samples of human origin. Other applications for use of this antibody have not yet been tested.Synonyms: ALOX15B, Arachidonate 15-lipoxygenase B, Arachidonate 15-Lipoxygenase Type II, 15-LO-2, 15-LOX-2, Linoleate 13-lipoxygenase 15-Lob. Immunogen: Synthetic peptide from the internal region of human 15-lipoxygenase-2. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Rabbit. Applications: ICC, IHC, WB. Cross Reactivity: (-) Human 5-Lipoxygenase, (-) 12-Lipoxygenase-1 (porcine leukocytes), (-) 15-Lipoxygenase-1 (rabbit reticulocyte). UniProt Accession #: O15296. Origin: Animal/Rabbit.