Human IgG F(ab')2 Rhodamine, Rhodamine Conjugated

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG, anti-Human IgG F(ab')2 and anti-Human Serum.  No reaction was observed against anti-Human IgG F(c) or anti-Pepsin. Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Human IgG F(ab')2 molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 molecules.