BIOMOL Online February 2006 1
 

February 2006

  
 


Featured Products
 SpeedLead-P - Phosphatase Selectivity
 Screening Service
 New Products
PTEN
 New Kinases
Angiogenesis Inhibitors
SUMOylation Kit
Featured Products


SpeedLead-PTM is a new selectivity screening service provided by BIOMOL and Innova Biosciences that provides accurate target selectivity data against a panel of highly active phosphatases.

% Inhibition Service - compounds are screened, in duplicate, against the panel at two concentrations

IC50 Service - 9 point dose-response analysis to follow up on interesting leads

We use rigorous and completely objective quality control and results are reported in 10 working days.

Enzyme Panel

PTP1B DEP-1
SHP-1 PTPRB
SHP-2 YOP51
LAR STP1
TC-PTP PTP-MEG
VHX VHR
IMPA-1 (Zn-activated) CD45
IMPA-2 (Mg-activated) CDC25A
Alkaline phosphatase PTP-PEST

new enzymes are in development
check for updates

Accelerate your drug discovery program with SpeedLead-P
Visit www.speedleadscreen.com for more information and to request a quotation.

In partnership with
New Products

PTEN
PTEN (Cat. # SE-402)
Human PTEN, full length with an N-terminal His-tag. PTEN  is one of the most common targets for mutation in sporadic human cancers and was the first phosphatase identified as a tumor suppressor. It is a lipid phosphatase which can also act as a tyrosine, serine and threonine protein phosphatase. Its main biological substrate, however, is phosphatidylinositol 3,4,5-triphosphate (PIP3, Cat.# PH-107).
Figure 1. Phosphate release by 500 ng of BIOMOL PTEN (SE-402) using PIP3 (PH-107) as a substrate. Phosphate released was detected using BIOMOL Green Reagent (AK-111)

For related products:

Phosphatases | Enzymes |  Assays |  Inhibitors |  Substrates

 

NEW KINASES
Sphingosine Kinase 1 (Cat. # SE-424) p38 gamma (Cat. # SE-426)
Sphingosine Kinase 2 (Cat. # SE-425) p38 delta (Cat. # SE-427)
MAPKAP3 (Cat. # SE-428) RSK1 (Cat. # SE-429)
DDR2 (Cat. # SE-430)

BIOMOL now offers over 90 recombinant active kinases.
View our complete offering online.
For related products:

Tyr Kinases | Ser/Thr Kinases | Lipid Kinases and Phosphatases |  Inhibitors | Phosphospecific Abs
 

ANGIOGENESIS INHIBITORS

BIOMOL Honors Judah Folkman with Lifetime Achievement Award

BIOMOL has sponsored a Lifetime Achievement Award to Judah Folkman,  Director of the Vascular Biology Program at Children’s Hospital Boston. Folkman’s work has largely founded the field of angiogenesis and has led to the discovery and implementation of angiogenesis inhibitors for the treatment of cancer and macular degeneration. For more on Judah Folkman, to request angiogenesis literature, or to view angiogenesis products, visit our angiogenesis page.

Fumagillin (Cat. # CT-100) - NEW LOW PRICE
An inhibitor of  methionine aminopeptidase 2 (MetAP2), which directly inhibits endothelial cell proliferation. Fumagillin analogs are being investigated as anti-cancer and anti-arthritic agents - inquire for availability.
Borrelidin (Cat. # CT-103)
 Displays pronounced anti-angiogenic activity (IC50=0.8 nM) and also induces the collapse of formed capillary tubes in a dose-dependent fashion.  In HUVECs the capillary tube collapsing activity is mediated by the induction of apoptosis.
Artesunate (Cat. PR-117)
Artesunate is a semisynthetic derivative of artemisinin, an antimalarial drug extracted from the plant Artemisia annua. Artesunate has been shown to inhibit angiogenesis in vivo and in vitro. Artesunate remarkably lowers VEGF expression in tumor cells and VEGF receptor expression on endothelial cells.
Terbinafine (Cat. # EI-318)
A CDK inhibitor that blocks HUVEC proliferation and capillary-like tube formation in chorioallantoic membrane assays.
3,5,4'-Trimethoxystilbene (Cat. # T-128)
A resveratrol analog that is 30- to 100-fold more potent in inhibiting endothelial cell proliferation, sprouting, collagen gel invasion and morphogenesis. (ID50=0.3-30 microM)
Imiquimod (Cat. # CT-106)
A topical immune modifier that upregulates IL-8, downregulates MMP-9 and inhibits angiogenesis.

Irsogladine maleate (Cat. # PD-141)
Selective PDE4 inhibitor that inhibits angiogenesis in wild-type and plasminogen activator-deficient mice.

Withaferin A (Cat. # CT-104)
A potent inhibitor of endothelial cell (HUVEC) sprouting (IC50 = 12 nM) and is active in vivo.  It alters cytoskeletal architecture by covalently binding annexin II and stimulating its basal F-actin cross-linking activity, which inhibits the migratory and invasive capability of endothelial cells.

For related products:

Angiogenesis Inhibitors | VEGF | HIF-1alpha | Sphingosine-1-phosphate | Proteases
 

SUMOylation Kit
SUMOylation Assay (Cat. # UW8955)
This assay kit contains all reagents necessary for the routine detection of SUMOylation activity. A mono-SUMOylation substrate (RanGAP1) is included as a control to allow clear detection of the SUMOylation reaction. Recombinant human SUMO E1 (Cat. # UW9330), recombinant human SUMO E2, (Cat. # UW9320), human SUMOs -1, -2, and -3 (Cat. #’s UW9195, UW9205, and UW9215), and antibodies to SUMO-1 (Cat. # PW8330) and SUMO-2/3 (Cat. # PW9465) are included in this kit. Sufficient for 20-50 assays.

For related products:

Antibodies to Ubl Proteins | Ubl Proteins | Ubl Protein Cascade Enzymes and Substrates 
 
Coming Soon
The BIOMOL 9th Edition Catalog/Handbook

The BIOMOL 9th Edition Catalog/Handbook will be available in March 2006. Reserve your copy today!

The BIOMOL Catalog/Handbook goes beyond the usual dictionary listing of reagents.  Reagents are introduced within specific signal transduction pathways organized by their respective function in the signaling cascade. Useful tables, application notes and up-to-date literature references for each reagent are also included.

 
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New Products
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Print Catalog
 Useful tables, application notes, and references

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 Peptides, antibodies and organic synthesis



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