BIOMOL Online November 2005
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November
2005
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| Featured
Products |
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InstaCellTM
- Cell-based Proliferation Assays |
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Frozen
cells INSTANTLY available for assay!
InstaCell Proliferation Assays allow fast and reliable
screening of drugs for their cytostatic or anti-cancer
effects. Well-characterized human tumor cells are frozen in
96 well plates providing ready-to-use and consistent cell
cultures instantly available for assay. No washing,
pre-cultivation or expansion steps are necessary. Kits
include four 96-well plates of cells, a fluorogenic or
chromogenic detection reagent, culture media, and a positive
control that blocks proliferation.
Cell lines:
MCF-7, SK-MEL, A549, HCT-116, HepG2, K562, BT-549.
Detection methods: Resazurin, Sulforhodamine B or
XTT.
Find
out more about the complete InstaCell range.
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Growth
inhibition curves of various InstaCell cell lines using
oxaliplatin |
NEDD8 (human, recombinant, His-tagged)
UW9225
NEDD8 is a ubiquitin-like molecule (sharing ~60% identity
with ubiquitin at the amino acid level) that can be
covalently conjugated (NEDDylation) to a limited number of
cellular proteins in a fashion similar to that of
ubiquitin. |
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ISG15 (human, recombinant, His-tagged)
UW9235
Cellular protein conjugates of ISG15 have been identified. ISGylation may play important roles in viral
or bacterial infection where protein ISGylation is highly
induced. |
FAT10
(human, recombinant, His-tagged)
UW9240
FAT10 is a ubiquitin-like protein that is encoded in the major histocompatibility complex class I locus.
Wild type FAT10 has been demonstrated to be covalently conjugated to target
proteins. |
Request
your FREE copy of our 'Ubiquitin and
Ubiquitin-like proteins' poster, as featured in
Nature Reviews Molecular Cell Biology.
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For related products:
BML-257 EI-336
Inhibits the translocation of Akt. The compound was discovered as a hit (12.5
microM) in a cell-based screen for Akt translocation inhibitors. |
BML-257
2,3-Diphenylquinoxaline-6-carboxylic acid
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Akt3 (human, recombinant)
SE-369
Useful for kinetic and functional studies, phosphorylation of target substrates, drug screening. |
Geldanamycin-biotin EI-341
A useful tool for affinity purification of HSP-90 and
HSP-90 dependent client proteins. |
Anti-alpha-Tubulin (K(Ac)40) SA-452
Acetylation of alpha-tubulin Lys40 has been shown to play a regulatory role in cell motility and possibly in dynein-mediated transport of misfolded proteins and aggresome function. |
Anti-SIRT3
SA-463
Caloric restriction elevates SIRT3 expression in mouse adipose
tissue and the absence of a SIRT3 allele lacking an enhancer element in long-lived
men suggests a possible connection between SIRT3 expression and longevity. |
 Western blot analysis of Jurkat cell lysate probed with Anti-SIRT3.
P and M indicate the
precursor (44 kDa) and mature (30 kDa) forms. |
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Anti-SIRT5
SA-464
SIRT5 is the only human class III sirtuin and as such is the closest homolog to bacterial (e.g.
E. coli CobB) and archaeal sirtuins. Antibody shows no cross-reactivity with other sirtuins tested (SIRTs 1, 2 and 3). |
 Western
blot analysis of PC12 cell lysate probed with Anti-SIRT5.
Arrow indicates position of mature SIRT5 at 29 kDa |
For related products:
Anti-Kallikrein-1 SA-447
Anti-Kallikrein-2 SA-448
Anti-Kallikrein-3
(PSA) SA-449
The kallikreins are a large family of secreted serine proteases with numerous functions and involved in many diseases.
Antibodies recognize the corresponding human kallikreins, whose size can vary greatly due to differential splicing,
post-translational modification, proteolytic cleavage, and covalent binding to endogenous inhibitors such as protein C
inhibitor. |
Immunoblot demonstrating cleavage of IGFBP-3 by ADAM12 during overnight incubation at 37°C.
Lanes 1,2: IGFBP-3 incubated alone; Lanes 3,4: IGFBP-3
with ADAM12.
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Tryptase,
betaII (human, recombinant) SE-418
Useful tool for studying enzyme kinetics, target substrate cleavage
and inhibitor screening. This preparation does not contain heparin, which can alter assay outcomes or interfere with inhibitors. |
ADAM12,
Catalytic Domain (human, recombinant)
SE-368
ADAM12
catalytic domain (residues 179-388) from human cDNA,
expressed in E.coli. Applications: study of enzyme
kinetics, cleave target substrates, and screen for
inhibitors. |
For related products:
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| Coming
Soon |
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The
BIOMOL 9th Edition Catalog/Handbook |
The BIOMOL 9th Edition Catalog/Handbook will be available in
January 2006. Reserve
your copy today!
The
BIOMOL Catalog/Handbook goes beyond the usual
dictionary listing of reagents. Reagents are
introduced within specific signal transduction pathways
organized by their respective function in the signaling
cascade. Useful tables, application notes and up-to-date
literature references for each reagent are also included.
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